Composite

Part:BBa_K3675005:Design

Designed by: Tiago Lopes   Group: iGEM20_NOVA_LxPortugal   (2020-10-22)


Prom2_GFP_T1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 607
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 676
    Illegal PstI site found at 607
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1195
    Illegal XhoI site found at 84
    Illegal XhoI site found at 1096
    Illegal XhoI site found at 1410
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 607
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 607
    Illegal NgoMIV site found at 1509
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1500
    Illegal SapI site found at 314
    Illegal SapI site found at 1457


Design Notes

The part corresponding to the promoter was altered to introduce the Shine-Delgarno sequence


Source

Besides the promoter sequence that was obtained using the online tool softberry (http://www.softberry.com/berry.phtml), both EGFP and terminator sequences were obtained in GenBank.

References